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Job Location | Edinburgh |
Education | Not Mentioned |
Salary | 30,000 - 40,000 per annum |
Industry | Not Mentioned |
Functional Area | Not Mentioned |
Job Type | Permanent , full-time |
The team are looking for an experienced scientist with a strong molecular biology background, with a particular focus on Next Generation Sequencing (NGS) to apply their technical experience and knowledge to a variety of client and company projects.Key Tasks:- The development, optimisation, and running of protocols to process samples for NGS (e.g. bulk-seq, single-cell RNA-seq, ATAC-seq). - Troubleshooting of library preparation and NGS readouts. - Actively interrogate scientific literature to understand new developments in NGS technologies.- Work within a team to ensure quality conduct, design and delivery of client projects (primarily relating to NGS and immunology).- Planning of projects in advance, arranging resources required for experimental conduct, ordering reagents etc.- Accurate record keeping of all experimental work, from initial protocol development, accurate lab notes, through to write up of results and report production.- Strong verbal communication skills, participating in lab and client discussions.- Implement quality control steps to ensure projects are conducted and delivered according to the standards expected, developing new or updating SOPs as required.- Data analysis and quantification using appropriate statistical methods.- May be required to train and line manage junior members of staff.Additional Tasks: - Production and maintenance of primary cell cultures (e.g., T cells, dendritic cells, macrophages) as well as various tumour cell lines as required.- Conduct experimental readout and analysis using cell sorting, flow cytometry, ELISAs, PCR.Desired Skills/Experience:- Life science degree (Biological Sciences/Molecular Biology/Oncology desirable), PhD an advantage.- Expertise in NGS library prep: bulk RNA-seq (Smartseq, TruSeq), single cell (10x genomics)- Laboratory experience in sterile TC methods, ELISAs, flow cytometry, RNA extraction and QC (use of Qubit and Bioanalyzer), targeted pre-amplification, Taqman qPCR.- Familiarity with single cell analysis and interpretation of gene expression and other single cell-omics data- Preferred: familiarity with single-cell PCR, VDJ sequence, nanostring, spatial transcriptomicsThey are looking for a dynamic individual to join their growing team, who would be excited about investigating novel compound activity on behalf of their clients.Salary entry level will be based on experience.