Cell Culture Research Scientist

Leading Cambridge based Contract Research Organisation seeks an experienced Cell Culture Research Scientist.Duties may include:

Employ careful aseptic technique and follow all tissue culture laboratory Standard Operating Procedures (SOPs) closely, keeping full and accurate details of procedures.
Maintain healthy cultures of stable heterologous and immortalized cell lines according to requirements.
Provide cells for electrophysiological assays on automated and manual patch clamp platforms as specified by the daily plan, liaising closely with staff and communicating any issues and observations to line manager.
Provide cells for plate-based assays, seeding plates at required density and pretreating cells and preparing plate wells as required by individual cell line protocols.
Prepare frozen stocks of assay-ready cells to facilitate screening on demand.
Scale-up supplies of cell line vials stored in liquid nitrogen banks, as requested.
Prepare cell culture reagents as required to support the electrophysiology team.
Preparation of reagents and solutions for electrophysiological experiments (this requires knowledge of Molarity calculation and pH correction of solutions).
Operate PCR Mycoplasma sp. screen as requested, and complete written and electronic records.
Carry out housekeeping duties in cell culture facility.
Administration duties to include stock inventory, placing of orders in online procurement system, unpacking and booking in deliveries on arrival.
Organization of service contracts for laboratory equipment.

Skills and experience:

BSc/MSc and/or at least three years of relevant laboratory experience.
Demonstratable lab-based experience in cell culture.
Excellent use of aseptic technique.
Experience with following SOPs.
Excellent attention to detail and ability to record and maintain accurate written records.
Be able to work in a fast-paced environment and adapt to changing priorities.
Proactive attitude and positive approach to help the team succeed.
Experience working in an industrial setting.
Familiarity of basic molecular biology techniques (e.g. PCR, Gel electrophoresis etc.).

Keyskills :
Cell CultureLaboratoryAssayAeptic

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